Optical clearing of ex vivo porcine dura mater by mannitol studied by confocal Raman micro-spectroscopy
Ali Jaafar,1,2,3 , Maxim E. Darvin4 , Ágnes N. Szokol1 , Valery V. Tuchin,4,5,6 and Miklos Veres1.
1 Institute for Solid State Physics and Optics, Wigner Research Center for Physics, P.O. Box 49, Budapest, H 1525, Hungary
2 Institute of Physics, University of Szeged, Dom ter 9, H-6720 Szeged, Hungary
3 Ministry of Higher Education and Scientific Research, 10065, Baghdad, Iraq
4 Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, 10117 Berlin, Germany
5Science Medical Center, Saratov State University, 83 Astrakhanskaya str., 410012 Saratov, Russia
7Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the Russian Academy of Sciences,” 24 Rabochaya str., 410028 Saratov, Russia
7А.N. Bach Institute of Biochemistry, FRC “Biotechnology of the Russian Academy of Sciences,” 33-2 Leninsky Prospect, 119071 Moscow, Russia
Abstract
Dura mater is a connective tissue with collagen fibrils architecture that protect the human brain. Tissue Optical clearing (TOC) technique was utilized to make DM more transparent using mannitol as an optical clearing agent, thereby permit to increase in-depth investigation by confocal Raman micro-spectroscopy. Mannitol is widely employed in cerebrovascular surgery as a measure in preventing necrosis and apoptosis of cells. Result show that collagen-related Raman band intensities were significantly increased for all depths in beginning of treatment and then decreasing for all depth after 2 and 3 min due to swelling of the tissue that occurs after the initial shrinkage of the tissue caused by mannitol. Furthermore, this method could be translated to other fibrous biological tissues and organs.
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ALI JAAFAR
Institute for Solid State Physics and Optics, Wigner Research Center for Physics, P.O. Box 49, Budapest, H-1525, Hungary
Hungary
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