Numerical Simulation of Light Propagation in Blood Layer and Estimation of Wavelength Dependence of Absorbed Light Portion
Stepan Goncharov1, Andrei Lugovtsov1, Alexander Priezzhev1; 1Lomonosov Moscow State University, Moscow, Russia
Abstract
In recent decades, promising results have been obtained demonstrating high efficiency of photobiostimulation (PBM) of living organisms. Many effects of PBM are associated with light interaction with blood. However, their mechanisms have not been fully explained yet. At the Laboratory of Biomedical Photonics of the Faculty of Physics of Lomonosov Moscow State University, studies are being carried out on the effect of in vitro laser irradiation of blood on its microrheological properties and, as a result, on its fluidity.
For the correct conducting of mesurements and interpretation of the results, it is necessary to estimate the portion of absorbed energy depending on the irradiation parameters and the physical state of blood (e.g the aggregation state of erythrocytes).
The aim of this work is to describe the model of blood sample and the process of its light irradiation, as well as to numerically simulate the propagation of a light beam in the sample, which makes it possible to estimate the irradiation dose absorbed by blood depending on light intensity and wavelenght.
The propagation of photons in the multiple-scattering medium was simulated using the original Monte Carlo code. The scattering phase functions were aproximated using the Mie and Henyey-Greenstein models.
The calculation results make it possible to relate the numbers of photons incoming, absorbed, and leaving the layer of blood, as well as their energy at different wavelengths (514, 633, and 1000 nm).
This makes it possible to compare the irradiation parameters with changes in the microrheological chracteristics of blood in experiments with different geometrical configurations of the irradiated sample at different wavelengths.
This work was supported by the Russian Science Foundation, Grant No. 26-15-00086.
Speaker
Goncharov Stepan
Faculty of Physics, Lomonosov Moscow State University
Russia
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