Study of microhemodynamic parameters and characteristics of erythrocytes during the development of pathology during photodynamic therapy with submicron nanoparticles
Ekaterina N. Lazareva1, Arseny P. Fashchevskii1, Artyom M. Mylnikov2, Nikita A. Navolokin1, 2, 3, Natalia V. Shushunova2, Irina Yu. Yanina1
1Saratov National Research State University named after N. G. Chernyshevsky, Saratov, Russia;
2Saratov State Medical University named after V. I. Razumovsky, Saratov, Russia;
3Pathomorphology Department of the State Healthcare Institution “Saratov City Clinical Hospital No. 1 named after Yu.Ya. Gordeeva", Saratov, Russia;
Abstract
Numerous blood components significantly influence its optical properties, and their contribution can change with the development of pathological conditions. Changes in blood composition during pathologies lead to changes in its optical properties, which can be used to develop new diagnostic methods for various diseases [1, 2]. Understanding the characteristics of changes in hemodynamics and blood microcirculation in tissues during pathological modeling plays an important role in preclinical testing of pharmacological drugs and will allow for a more meaningful interpretation of research results. The impact of pathology on tissue circulation remains poorly understood. Therefore, analysis of blood flow parameters and erythrocyte morphometry is not only a diagnostic marker but also reflects key links in the pathogenesis of cancer [3, 4].
This work presents blood flow parameters and characteristics of red blood cells during the development of pathology during photodynamic therapy (PDT) using submicron nanoparticles. The presented results of studying microdynamic parameters using Doppler ultrasonography and microscopic blood examinations revealed that irreversible physiological changes occur during tumor tissue development, which are influenced by PDT using submicron nanoparticles. Analysis of rat blood flow parameters revealed that, in the early stages of tumor development, increases in heart rate, maximum blood flow velocity, average blood flow velocity, maximum pressure gradient, and average pressure gradient are observed. Analysis of red blood cell parameters obtained from rat whole blood revealed that the development of pathology significantly alters the tissue and leads to a decrease in red blood cell area. Comparison of results on different days of tumor tissue development with and without PDT revealed that changes are observed already in the early stages of tumor formation. The obtained data serve as a basis for further research and will help in studying the effects of photodynamic therapy using submicron nanoparticles on blood flow microdynamics.
The work was supported by the grant of the Russian Science Foundation No. 25-22-00144.
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Ekaterina N. Lazareva
Saratov State University, Saratov, Russia
Russia
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