Mesoscopic fluorescence molecular tomography: A numerical study of the optical clearing effect
Alexander B. Konovalov1, Vitaly V. Vlasov1, Sergei I. Samarin1, Daria K. Tuchina2,3, Alexander P. Savitsky,4,5 Olga P. Cherkasova,5 Valery V. Tuchin2,3,6
1 FSUE “Russian Federal Nuclear Center – Zababakhin All-Russia Research Institute of Technical Physics,” Snezhinsk, Russia
2 Institute of Physics and Science Medical Center, Saratov State University, Saratov, Russia
3 Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russia
4A.N. Bach Institute of Biochemistry, Federal Research Centre ‘Fundamentals of Biotechnology’ of the Russian Academy of Sciences, Moscow, Russia
5National Research Centre «Kurchatov Institute», Moscow, Russia
6Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the RAS”, Saratov, Russia
Abstract
The paper is devoted to the study of the tissue optical clearing effect through numerical experiment on the reconstruction of a phantom with a fluorophore with the use of mesoscopic fluorescence molecular tomography (mFMT). It is the first attempt to answer the question on whether it is possible to improve the reproduction accuracy of spatial structures and mFMT depth sensitivity through the optical clearing of the object surface. The reconstruction region is a cube 10x10x10 mm3 in size. The fluorophore is specified as two nested cylindrical shells 0.5 mm thick at a distance of 0.5 mm between them. This allows us to estimate the modulation transfer coefficient for structures 0.5 mm in size for the entire depth of the object. Fluorescence signals and sensitivity functions are modeled in reflectance geometry by the Monte Carlo method with the TurbidMC code. The inverse problem is solved using the algebraic reconstruction technique with the total variation regularization. Reconstruction results demonstrate that optical clearing can help increase from 5 to 7 mm the depth available for the accurate reproduction of the structures.
The investigation was supported by the Russian Science Foundation with Grant No. 26-63-00028,
https://rscf.ru/en/project/26-63-00028/.
Speaker
Alexander B. Konovalov
FSUE “Russian Federal Nuclear Center – Zababakhin All-Russia Research Institute of Technical Physics,” Snezhinsk, Russia
Russia
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