Visualization of cardiac-related perfusion oscillations using a laparoscopic laser speckle contrast imaging system
Vadim N. Prizemin, Nadezda V. Golubova, VIktor V. Dremin, Elena V. Potapova; Orel State University named after I.S. Turgenev, Orel, Russia
Abstract
Laser speckle contrast imaging (LSCI) is a non-contact optical method that enables real-time visualization of tissue microcirculation. Integration of LSCI into a laparoscopic system may extend the capabilities of minimally invasive surgery by providing intraoperative information on both the spatial distribution and temporal dynamics of blood flow. This study presents a real-time processing approach for simultaneous visualization of speckle perfusion and cardiac-related microcirculatory oscillations.
Previously acquired experimental video sequences were processed as if they were received directly from the laparoscopic imaging system. Speckle contrast was calculated using spatial and temporal averaging, followed by conversion into a relative speckle perfusion index. The temporal variations of the perfusion signal were analyzed using the fast Fourier transform within a continuously updated sliding window. The dominant spectral component within the expected cardiac frequency range was identified, and its amplitude was used to visualize the spatial distribution of cardiac-related perfusion oscillations.
The proposed approach was evaluated using recordings obtained from intact laboratory animals and animals after experimentally induced hemorrhagic shock. Additional measurements were performed on peripheral tissues before and during limb occlusion. The results demonstrated changes in both the overall perfusion level and the amplitude of cardiac-related oscillations under systemic and local blood flow disturbances.
The developed algorithm enables simultaneous real-time assessment of tissue perfusion and its pulsatile component. This combined analysis may improve the detection of hemodynamic alterations and enhance the functional capabilities of laparoscopic LSCI systems for experimental and intraoperative applications.
Speaker
Vadim Prizemin
Orel State University named after I.S. Turgenev, Orel, Russia
Russia
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