Development and Characterization of a Near-Infrared Fluorescent Renca Cell Line for Preclinical Renal Cell Carcinoma Research
Elizaveta Petrova 1*, Daria Artamonova 1, Alexandra Bruter 2, Olga I Gusliakova 3, Artem Isaev 1, Gleb B. Sukhorukov 1. Olga A. Sindeeva 1; 1Skolkovo Institute of Science and Technology, Moscow, Russia; 2Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia; 3Science Medical Center, Saratov State University, 83 Astrakhanskaya Street, Saratov, Russia.
Abstract
Renal cell carcinoma (RCC) accounts for approximately 2% of all cancer diagnoses and is the 12th most common malignancy worldwide. A variety of cell lines is available for RCC research, among which the Renca cell line is one of the most widely used. Derived from a spontaneous murine renal adenocarcinoma, Renca cells may be implanted orthotopically. The resulting tumors resemble aggressive RCC and have the potential to metastasize to the lungs. Tumor growth and metastatic spread in this model are typically monitored using bioluminescence imaging. Although this technique is suitable for longitudinal in vivo studies, its application to ex vivo tissue imaging and histological analyses is limited. To address these limitations, we generated a Renca cell line that expresses miRFP720, which is one of the brightest near-infrared fluorescent proteins that are currently available. We demonstrate that Renca-miRFP720 cells retain the characteristics of the original cell line, including morphology and proliferation, and form orthotopic tumors with preserved metastatic potential. Importantly, both primary tumors and lung metastases can be readily detected by fluorescence imaging, which facilitates ex vivo tissue imaging and histological analysis. This model represents a valuable tool for preclinical studies of RCC progression.
Speaker
Elizaveta Petrova
Skolkovo Institute of Science and Technology
Russia
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