Comparison of morphological changes in organs and tumors in mice bearing CT-26 tumors and rats bearing PC-1 tumors during photodynamic therapy with a submicron-scale complex.
Artyom M. Mylnikov1, Nikita A. Navolokin1, Irina Yu. Yanina2
1Saratov State Medical University, Saratov
2Saratov State University, Saratov
Abstract
Comparison of morphological changes in organs and tumors in mice bearing CT-26 tumors and rats bearing PC-1 tumors during photodynamic therapy with a submicron-scale complex.
The aim of the study was to compare morphological changes in internal organs and tumor tissue in mice bearing CT-26 tumors and rats bearing PC-1 tumors after administration of the submicron-scale complex NaYF4+HSA+Cy3, irradiation, and a combination of both.
Materials and Methods. Histological examination of the liver, spleen, lungs, kidneys, myocardium, and tumors of experimental animals was performed. Each model included a comparison group, a group receiving the complex, a group receiving irradiation, and a group receiving the combined treatment. Photodynamic therapy was performed using a 980 nm laser.
Results. In both models, vascular changes predominated in the internal organs, manifested by varying degrees of congestion, moderate myocardial edema, and isolated alterative changes in hepatocytes. No irreversible damage or necrosis was detected in the examined organs. In mice treated with CT-26, the proportion of tumor necrosis in the comparison group was 10–20%, compared to 10–20% after intravenous administration of the complex, up to 30% after irradiation, and approximately 20% with a combination of the complex and irradiation. In rats treated with PC-1, necrosis in the control tumor accounted for 10–20%, up to 30% after complex administration, 30–40% after irradiation, and 40–50% of the sectioned area with the combined effect.
Conclusion. The morphological response of PC-1 tumors was more pronounced compared to CT-26, especially with PDT using the submicron-scale complex. Differences in efficacy may be associated with the biological sensitivity of tumors, features of the experimental design, blood supply, and accumulation of nanoparticles in tumor tissue.
Speaker
Artyom M. Mylnikov
Saratov State Medical University
Россия
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