Assessment of Peripheral Hemodynamics by Diffuse Reflectance Spectroscopy in the Visible and Near-Infrared Range in the Cuprizone-Induced Demyelination Model
Veronika Nikolaevna Volodina¹, Anastasia Andreevna Chervakova¹, Alexander Vladimirovich Kolpakov², Diana Yurievna Sokolova², Petr Evgenievich Zaitsev¹, Ulyana Aleksandrovna Apukhtina¹, Tatiana Ivanovna Yaremenko¹, Mikhail Sergeevich Krasnov¹, Nikolai Nikolaevich Kuznetsov¹,³, Viktoria Vyacheslavovna Zherdeva¹
¹A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia
²Faculty of Biomedical Engineering, Department of Biomedical Technical Systems, Bauman Moscow State Technical University, Moscow, Russia
³FN 5 “Chemistry”, Bauman Moscow State Technical University, Moscow, Russia
Abstract
Introduction. Cuprizone-induced demyelination in mice is a widely used model of multiple sclerosis, enabling the study of primary mechanisms of myelin damage. Previously, we observed that animals receiving cuprizone demonstrated a statistically significant (p < 0.05) prolongation of recovery time after inhalation anesthesia compared to the control group: the mean awakening time was 10 min versus 2–5 min in controls, which may indicate systemic hemodynamic or metabolic disturbances associated with demyelination.
The aim of the present study was to evaluate the feasibility of using diffuse reflectance spectroscopy in the visible and near infrared range for non invasive monitoring of peripheral circulation and tissue oxygenation in mice with cuprizone induced demyelination.
Materials and Methods. The study was performed on female C57BL/6 mice (n = 20), divided into two groups: a control group (standard diet) and an experimental group (diet supplemented with 0.4% cuprizone for 90 days). To assess peripheral hemodynamics, near infrared diffuse reflectance spectroscopy was employed in the wavelength range of 530–1000 nm, with spectra recorded from the surface of the tail vein. The informativeness of this spectral range for evaluating tissue oxygen saturation is determined by the absorption spectrum characteristics, as well as by the presence of isosbestic points of oxy and deoxyhemoglobin at wavelengths around 550 nm and 800 nm. The spectra were processed using the moving average method (17 points) to smooth noise.
Results. Diffuse reflectance spectra revealed statistically significant differences between the control and experimental groups. In cuprizone-treated mice, a change in the spectral curve pattern was observed, manifested as a decrease in the reflected signal intensity in the 550–600 nm range, which may reflect alterations in hemoglobin concentration or oxygenation in peripheral vessels. The prolonged recovery time from anesthesia observed in cuprizone-fed mice may result from both central mechanisms (impaired neurotransmission, mitochondrial dysfunction) and peripheral mechanisms (changes in peripheral blood flow and tissue oxygenation). Thus, diffuse reflectance spectroscopy in the visible and near infrared range may serve as an additional non invasive method for assessing systemic hemodynamic changes in demyelination models.
Conclusion. The use of diffuse reflectance spectroscopy in the visible and near infrared range enables non invasive registration of changes in peripheral blood circulation and tissue oxygenation in mice with cuprizone induced demyelination, correlating with the clinically observed prolongation of recovery time after anesthesia. The obtained results open up prospects for employing this method for monitoring systemic hemodynamic disturbances in preclinical models of neurodegenerative diseases.
Acknowledgements. The work was carried out under the State Assignment of the Ministry of Science and Higher Education of the Russian Federation.
Speaker
Veronika N. Volodina
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia
Russia
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