Optical stimulation of nerve cell growth on nanoparticles arrays
Uliana E. Kurilova1,2, Denis V. Novikov1, Yulia V. Chumachenko1, Andrey M. Tarasov1, Sergey V. Dubkov1, Dmitry G. Gromov1, Irina A. Suetina3, Marina V. Mezentseva3, A.Yu. Gerasimenko1,2; 1National Research University "MIET", Moscow, Russia; 2Sechenov First Moscow State Medical University, Moscow, Russia; 3National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F. Gamaleya, Moscow, Russia
Abstract
Nowadays the development of noninvasive methods for neuromodulation is an important problem in biomedicine. Gold and silver nanoparticles, which exhibit localized surface plasmon resonance, are capable of converting light into localized physicochemical signals, opening the way to optical stimulation of nerve cells. In this study, we investigated the effect of green light irradiation on the growth of Neuro-2a cells cultured on substrates of arrays with immobilized Au and Ag nanoparticles with different virtual thicknesses.
Nanoparticle arrays were formed using vacuum-thermal evaporation and subsequent rapid thermal annealing on glass substrates. The nanoparticles were irradiated with a continuous laser beam at a wavelength of 532 nm for 72 hours. Cell morphology and neurite length were evaluated with microscopy after 24 and 72 hours of irradiation. Irradiation of cells cultured on plasmonic nanoparticle substrates was shown to increase the average length and number of neurites compared to control groups. This effect was observed after 24 hours of culture. The greatest effect was observed for gold nanoparticles with virtual film thicknesses of 5 and 10 nm. These results demonstrate the potential of plasmonic-optical interfaces for targeted stimulation of neuronal cells, controlled by the placement of nanoparticles on the substrate.
The work was carried out as part of a major scientific project of the Ministry of Science and Higher Education of the Russian Federation, Agreement No. 075-15-2024-555 dated April 25, 2024.
Speaker
Uliana Kurilova
National Research University "MIET", Sechenov First Moscow State Medical University
Russia
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