Nonlinear optical microscopy for biomedical applications
Nirmal Mazumder
Department of Biophysics, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
Abstract
A Stokes polarimeter was build using four-channel photon counting detectors and integrated with second harmonic generation (SHG) microscopy for characterizing polarization properties of light. The Stokes–Mueller-based polarization technique enables detailed characterization of both SH light and sample properties. This system allows for the simultaneous measurement of polarization states and key polarization parameters without requiring repeated image acquisition. Two-dimensional polarization imaging facilitates in-depth investigation of molecular orientation in targeted specimens such as collagen fibers, skeletal muscle fibers, and starch granules. Additionally, a four-channel Stokes–Mueller polarimetry system integrated with machine learning has been developed in linear optics for the classification and characterization of ductal carcinoma tissue. This instrument offers a cost-effective solution for quantitative polarimetry in microstructural analysis of breast tissue samples. Various polarization parameters are derived from Stokes–Mueller polarimetry; notably, under circularly polarized incident light, higher degree of polarization values are observed. Integrating Stokes vector-based polarimetry with microscopy and machine learning provides powerful insights for detailed microstructural analysis of tissues and holds significant potential as a diagnostic tool.
Speaker
Dr. Nirmal Mazumder
Manipal Academy of Higher Education, India
India
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