Indocyanine Green-Mediated Photodynamic Therapy in Combined Chemotherapy of TSCC Cancer Model
Afshan Shirkavand1*, Sogol Saberi2, Fedora Khatibi Shahidi3, Sima Shahabi4
1. PhD, Assistant Prof of Biophotonics/ Medical Physics, Photodynamic department, Medical Laser Research center, YARA institute, ACECR, Tehran, Iran.
2. DDS, PhD, Assistant Prof, Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
3. PhD candidate in Biochemistry, Researcher, Photodynamic Department, Medical Laser Research Center, YARA Institute, ACECR, Tehran, Iran.
4. DDS, PhD, Prof of Dentistry, Department of Dental Biomaterials, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
Among cancers, oral squamous cell carcinoma (OSCC), accounts for 1-2% worldwide which has a high incidence of invasion of the surrounding tissues. Tongue cancer is the most prevalent subtype of oral cancer which remains difficult to manage because of its aggressive behavior, frequent recurrence, and incomplete responses to conventional treatment. We conducted an in vitro study based on the indocyanine green (ICG)-mediated photodynamic therapy (PDT) in combination with Cisplatin chemotherapy to investigated the effect of ICG-mediated PDT on enhancing the anticancer activity of cisplatin in TSCC-1 cells. We focused on apoptosis and cell migration in dark and irradiated circumstances due to the fluorescence effect of ICG as photosensitizer.
Cells cultured based on the standard protocol. Then, were incubated using ICG, and irradiated using a 660 nm LED, and treated by Cisplatin. Cell apoptosis, and migration were assessed using AO/EB staining, and scratch assay. The combined ICG-PDT and cisplatin treatment produced increased apoptosis, and reduced migration in cancer cells. Quantitative interaction analysis using Bliss independence and Chou-Talalay Combination Index further indicated a dose-dependent response, with greater-than-additive/synergistic interaction at selected low-to-intermediate dose combinations. Our findings support ICG-PDT combination therapy in a TSCC cancer model for more optimized light-drug dosing to reduce the cisplatin burden while preserving anticancer efficacy.
Key words: TSCC, Cancer, Photodynamic, Conventional treatment, ICG photosensitizer
Speaker
Afshan Shirkavand
Assistant Prof of Biophotonics/ Medical Physics, Photodynamic department, Medical Laser Research center, YARA institute, ACECR, Tehran
Iran
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