Biophysical Differences Between Macrophages of Distinct Origin
Dmitry Kolesov1,2, Aleksandr Vaneev2, Vasiliy Kolmogorov2, Aida Bagdasaryan3, Antonina Illarionova3, Polina A. Vishnyakova3
1. Moscow Institute of Physics and Technology, Moscow, Russia
2. NUST MISIS, Moscow, Russia
3. RUDN University, Moscow, Russia
Abstract
Macrophage activation by lipopolysaccharide (LPS) involves profound biochemical and biophysical remodeling, yet nanoscale mechanical and oxidative changes accompanying CD14++ and CD16++ phenotyping remain poorly resolved. Scanning ion-conductance microscopy (SICM) offers a non-contact, high-resolution approach for simultaneously probing cell topography, stiffness, and, when coupled with functionalized nanopipettes, local reactive oxygen species (ROS). This study employed SICM to quantify stiffness and ROS production in macrophages exposed to LPS and stratified by CD14++ and CD16++ expression. SICM imaging revealed that LPS exposure significantly increase cellular stiffness both for CD14++ and CD16++ macrophages. Measurements using carbon nanoelectrodes showed that the level of ROS production by CD16++ macrophages upon addition of LPS was higher. These findings establish SICM as versatile tool for dissecting mechano-inflammatory coupling in innate immune cells, with implications for understanding and modulating macrophage function in inflammatory diseases.
Speaker
Dmitry Kolesov
Laboratory of medical equipment in the field of in vitro diagnostics, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow region, Russia
Russia
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