Two‑photon microscopy as a method for studying the biocompatibility of medical implants.

Abstract

Currently, a large number of materials for osseointegration are being produced. They must meet the parameters of biocompatibility, such as low cytotoxicity and good integration with the body. Titanium nickelide is one of the most common medical materials and is one of the most sought‑after materials for implantation. One way to test the biocompatibility of titanium nickelide is to use the two‑photon microscopy, which allows one to determine the fluorescence lifetime of the endogenous fluorophore NAD(P)H and make a conclusions about the metabolism of the cells cultured in the presence of an implant. In this study, cellular metabolism of fibroblasts incubated on titanium nickelide samples was analyzed using two‑photon microscopy with a laser pump wavelength of 760 nm. It was concluded that cellular metabolism may shift both towards oxidative phosphorylation, which is responsible for the normal vital activity of cells, and towards glycolysis during the culture process. A shift in cellular metabolism towards glycolysis may indicate that the cells may experience a state of hypoxia during incubation on porous titanium nickelide samples. This may be due to the fact that the cells have penetrated into the pores of the samples and are experiencing a lack of oxygen. Glycolysis increases to compensate for the reduced activity of mitochondrial respiration under hypoxic conditions. Thus, this work has demonstrated the potential for using two‑photon microscopy as a method for analyzing the biocompatibility of biomedical implants.
The investigation was supported by Russian Science Foundation with the grant № 24-63-00049, https://rscf.ru/project/24-63-00049/.

Speaker

Lepekhina Tatiana Borisovna
Tomsk State University, Tomsk, Russia
Russia

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