Investigation of the effect of upconversion nanoparticles on the optical properties of tumor tissue. Prospects for PDT
Viсtoriia A. Kovalenko¹, Nikita .A Navolokin², Artyom M . Mylnikov², Irina Yu .Yanina¹
¹Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russian Federation.
²Saratov State Medical University named after V.I. Razumovsky, 112, B. Kazachya St.,Saratov410012, Russian Federation.
Abstract
Photodynamic therapy is currently an actively developing method for treating malignant tumors. The attractiveness of the method lies in the possibility of destroying tumor cells using low-power laser exposure, which minimizes the risk of unwanted damage to healthy areas. To minimize the likelihood of systemic side effects, upconversion nanoparticles (UCNPs) are considered as a means of drug delivery.
During the study, a solution of UCNPs NaYF4 coated with human serum albumin (HSA) and Cy3 dye (concentration 2 mg/ml) was applied to a tumor section (cholangiocarcinoma line PC-1). Then the tumor was exposed to laser irradiation for 1, 3 and 5 minutes using a semiconductor laser LSR980NL-FC-1W. At different stages of the experiment, diffuse transmission and reflection spectra were detected in the range of 350-1500 nm, and the refractive index of tumor tissue samples was measured in the spectral region of 480-1550 nm. As a result of the study, changes in the optical properties of tumor tissue during the procedure were analyzed.
The study was supported by a grant Russian Science Foundation No. 25-22-00144,
https://rscf.ru/project/25-22-00144/.
Speaker
Viktoriia Kovalenko
Saratov State University
Russia
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