Study of the influence of tumor tissue development on blood flow using Dopplerography and microscopy methods
Lazareva E.N.1, Fashchevsky A.P.1, Mylnikov A.M2, Navolokin N.A.1,2,3, Yanina I.Yu.1,4; 1Saratov State University, Saratov, Russia; 2V. I. Razumovsky Saratov State Medical University, Saratov, Russia; 3Yu.Ya. Gordeev Saratov city clinical hospital No. 1, Pathomorphology department, Saratov, Russia; 4Tomsk State University, Laser Molecular Imaging and Machine Learning Laboratory, Tomsk, Russia
Abstract
Currently, a pressing problem in medicine is the diagnosis of cancerous tumors at early stages, when therapeutic treatment is most effective. It is well known from the literature that during the development of tumor tissue, changes in the circulatory system occur, concerning, for example, the biochemical composition, shape and content of formed elements of the blood [1-3]. In this work, the study of the rat blood supply system was carried out on the DUB SkinScanner ultrasound device (tpm taberna pro medicum, Germany). The blood flow parameters were assessed using the DUB SKINSCANNER DOPPLER software. During the study, the following blood flow parameters were determined for the control (healthy) group of animals and for the group of animals with transplanted alveolar liver cancer: heart rate (HR), peak systolic blood flow velocity (PSV - Peak Systolic Velocity), average blood flow velocity (Vel_mean), maximum systolic pressure gradient (PG_PSV), average systolic pressure gradient (PG_mean), pulsatility index (PI, PI Pulsatility Index), resistance index (RI). An additional research method was microscopy, which was used to evaluate the size and shape of red blood cells. The results of the work showed that the development of tumor tissue causes irreversible changes in the circulatory system, which can be determined at early stages and can serve as an indicator of the development of pathology.
The study was supported by the grant of the Russian Science Foundation No. 25-22-00144,
https://rscf.ru/project/25-22-00144/
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[2] Li W. et al. High-resolution magnetic resonance angiography of tumor vasculatures with an interlocking contrast agent //ACS nano. – 2024. – Т. 18. – №. 37. – С. 25647-25656.
[3] Verkhovskii R. A. et al. Cytotoxicity of various types of coated upconversion nanoparticles. Overview //Izvestiya of Saratov University. Physics. – 2022. – Т. 22. – №. 4. – С. 357-373.
Speaker
Ekaterina N. Lazareva
Saratov State University
Russia
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