Diffusion coefficient measurements in ex vivo murine brain tissue via fluorescence recovery after photobleaching
A. I. Dubrovsky1, A. V. Dmitrenko2, I. V. Fedosov1, O. V. Semyachkina-Glushkovskaya2, S. B. Venig1; 1Institute of Physics, Saratov State University, Saratov, Russia; 2Department of Biology, Saratov State University, Saratov, Russia
Abstract
The molecular transport in the brain tissue is yet to be fully studied and presents great interest to the researchers in the fields of biophysics and biomedicine. The objective of this research was to develop a method for ex vivo quantitative measurements of diffusion coefficient in the ex vivo brain tissues of laboratory mice. Multiphoton microscopy-based fluorescence recovery after photobleaching was applied in the analysis of fluorescent dye molecular mobility dynamics in mouse brain tissue. The results of the fluorescence recovery dynamics analysis in an ex vivo slab of the mouse brain were used for molecular diffusion mapping in a murine brain tissue layer. Diffusion coefficient values in several areas of the brain tissue were calculated based on a simplified diffusion model and fluorescence recovery after photobleaching data. Fluorescence recovery after photobleaching with multiphoton microscopy were applicable in the creation of the molecular diffusion map in the brain tissue of a laboratory mouse.
This work was supported by the Russian Science Foundation (Grant No. 23-75-30001).
Speaker
Alexander Dubrovsky
Saratov State University
Russia
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