Study of the interaction of optical clearing agents with tissue phantoms based on synthetic opals
Arsenii P. Fashchevskii1, Yuriy I. Surkov1, Isabella A. Serebryakova1, Arsen K. Zotov2, Anna S. Kycheryavenko3, Gleb M. Katyba3, Valery V, Tuchin1,4,5
1Saratov State University, Saratov, Russia
2Prokhorov General Physics Institute, Moscow, Russia
3Osipyan Institute of Solid State Physics RAS, Chernogolovka, Russia
4Tomsk State University, Tomsk, Russia
5Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the RAS,” Saratov, Russia
Abstract
Measurement of the refractive index (RI) kinetics of an optical clearing agent (OCA) during the optical clearing (OC) process is an important task in biophotonics, providing deeper insight into the mechanisms of agent penetration and distribution in complex media. In this work, we present a methodology that enables high-precision determination of this parameter. As OCAs, 70% and 90% aqueous solutions of glycerol were used. The objects of study were synthetic opals (SO) — porous matrix structures composed of densely packed monodisperse silica (SiO₂) spheres.
The method is based on recording temporal changes in the optical thickness of SO using optical coherence tomography (OCT). In addition, mass measurements of the sample are performed in three states: in the dry form, after saturation with distilled water, and after saturation with the OCA. These measurements allow the calculation of the RI kinetics of the OCA.
The obtained time dependence of the OCA’s RI also makes it possible to determine physical parameters of the medium in which the OC process takes place, such as geometric thickness, porosity, and RI.
To evaluate the accuracy of the obtained data, the proposed methodology was tested. For comparison, the refractive index matching method was applied, as well as a simpler reference sample with precisely known porosity and uniform geometric thickness.
This study was supported by the grant from the Russian Sciece Foundation № 23-14-00287
Speaker
Arsenii Fashchevskii
Saratov State University, Saratov, Russia
Russia
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