Effect of riboflavin and blue laser radiation on cell death of melanoma cells B16F10

Abstract

Cutaneous melanoma remains one of the most aggressive and treatment-resistant forms of malignant neoplasms. Current approaches to treatment cutaneous require the development of novel, targeted methods. Photodynamic therapy (PDT) is a promising strategy when a photosensitizer is activated by light, which cause the formation of reactive oxygen species and lead to the death of tumor cells. Riboflavin (vitamin B2) is widely known biocompatible photosensitizer that can be activated by blue light. That fact makes riboflavin a suitable candidate for PDT.
The aim of this research is to evaluate the effects of laser radiation (450 nm) and riboflavin, and their combined effects, on the induction of cell death in the B16F10 melanoma cell culture.
In our experiments, we study the effects of laser radiation at a wavelength of 450 nm on the apoptosis and necrosis in riboflavin-photosensitized B16F10 melanoma cells.
We found that laser radiation and riboflavin as a model of photodynamic therapy, both separately and in combination, cause cell death in melanoma cells. The concentration of riboflavin plays a significant role in cell death mechanisms. Also, the cytotoxic effects of non-photosensitized riboflavin on melanoma cells were defined.

Speaker

Aigul Gilmutdinova
Ulyanovsk State University, Ulyanovsk
Russia

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