Antioxidant defence system dynamics in human fibroblast spheroids after continuous wave 1265 nm laser irradiation
Anna Khokhlova1, Irina Kolodii1, Valeria Ribenek1, Yury Saenko1, Elena Yurova1, Aigul Gilmutdinova1, Sergei Sokolovski2, Edik Rafailov2, Dmitrii Stoliarov2, Dmitriy Korobko1, and Andrei Fotiadi1,3;
1S.P. Kapitsa Scientific Technological Research Institute, Ulyanovsk State University, 42 Leo Tolstoy Str., 432970 Ulyanovsk, Russia;
2Optoelectronics and Biomedical Photonics Group, School of Engineering and Applied Science, Aston University, Birmingham B4 7ET, UK;
3Electromagnetism and Telecommunication Department, University of Mons, B-7000 Mons, Belgium
Abstract
Laser radiation in the 1264–1270 nm spectral range has recently gained attention as a promising tool in the treatment of malignancies due to its ability to induce photodynamic effects in living cells without the use of exogenous photosensitizers. However, the underlying mechanisms of action, particularly regarding its impact on normal cells and tumour-adjacent healthy tissues, remain insufficiently characterised. Three-dimensional (3D) fibroblast cultures offer a physiologically relevant in vitro model to study tumour microenvironment dynamics. Fibroblasts are critical components of the extracellular matrix and are present both in multicellular tumour spheroids and within the native tissue stroma. Therefore, understanding the photochemical effects of 1265 nm laser exposure on fibroblast spheroids is essential for the safe and effective development of photosensitizer-free photodynamic therapy protocols. In this study, we evaluate the antioxidant defence responses in 3D spheroids derived from normal human skin fibroblasts following exposure to continuous-wave (CW) laser radiation at 1265 nm. We specifically assess the dynamics of key antioxidant enzymes, including superoxide dismutase (SOD), catalase, and the glutathione system, along with the accumulation of lipid peroxidation products. These parameters provide insights into the redox balance and oxidative stress modulation in normal tissue models under near-infrared laser irradiation.
The work is supported by the Russian Science Foundation (grant number 23-79-30017) and the Ministry of Science and Higher Education of the Russian Federation (project FEUF-2023-0003).
Speaker
Anna Khokhlova
S.P. Kapitsa Scientific Technological Research Institute, Ulyanovsk State University, Ulyanovskiversity, Ulyanovsk
Russia
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