Comparative photobiological effects of laser radiation in 2D and 3D cell cultures
Anna Khokhlova1, Irina Kolodii1, Valeria Ribenek1, Yury Saenko1, Aigul Gilmutdinova1, Sergei Sokolovski2, Edik Rafailov2, Dmitrii Stoliarov2, Dmitriy Korobko1, and Andrei Fotiadi1,3;
1S.P. Kapitsa Scientific Technological Research Institute, Ulyanovsk State University, 42 Leo Tolstoy Str., 432970 Ulyanovsk, Russia;
2Optoelectronics and Biomedical Photonics Group, School of Engineering and Applied Science, Aston University, Birmingham B4 7ET, UK;
3Electromagnetism and Telecommunication Department, University of Mons, B-7000 Mons, Belgium
Abstract
The transition from conventional monolayer cell cultures to preclinical animal models presents significant challenges, including ethical concerns and limited translational relevance. Notably, data obtained from 2D cell cultures often fail to accurately reflect biological responses within whole organisms. In this context, three-dimensional (3D) cell cultures have emerged as more physiologically relevant in vitro models, particularly valuable in oncology research. These models better mimic the early stages of tumour development, where therapeutic modalities such as photodynamic and laser-based treatments are most effective. Despite growing interest in photobiomodulation and laser therapies, current understanding of the photobiological effects induced by laser irradiation in 3D tumour models remains limited—especially across varying wavelengths and biological contexts. In this study, we investigate the impact of continuous-wave laser radiation in the visible (450 nm and 650 nm) and near-infrared (1265 nm) spectral ranges on spheroid formation, growth dynamics, and viability. We employ 3D cultures generated from human melanoma (A875), colon adenocarcinoma (HCT116) cell lines, and normal human skin fibroblasts (HFS) to evaluate wavelength-specific effects and cellular responses.
The work is supported by the Russian Science Foundation (grant number 23-79-30017) and the Ministry of Science and Higher Education of the Russian Federation (project FEUF-2023-0003).
Speaker
Anna Khokhlova
S.P. Kapitsa Scientific Technological Research Institute, Ulyanovsk State University, Ulyanovsk
Russia
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