Optimization of Metal-Enhanced Fluorescence of Self-Similar Colloidal Nanoantennas for Single Molecule Detection
Sergey V. Zarkov1,2, Yuri A. Avetisyan1, Garif G. Akchurin1,3, Alexander N. Yakunin1, Valery V. Tuchin1,3,4; 1Institute of Precision Mechanics and Control, Federal Research Centre “Saratov Scientific Centre of the Russian Academy of Sciences”, Saratov, Russia;
2.Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia;3.Department of Optics and Biophotonics and Science Medical Center, Saratov State University, Saratov, Russia; 4Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russia
Abstract
Currently, the direction of single-molecule fluorescence spectroscopy is actively developing, which allows studying the dynamics of proteins and DNA, as well as developing methods for sequencing individual DNA molecules. Enhancement of the fluorescence response for single-molecule detection using a self-similar colloidal gold nanoantenna was experimentally demonstrated in by L. Novotny et al "Self-Similar Colloidal Antennas for Cascaded Field". Phys. Rev. Lett., 2012, vol. 109, art. 017402. . We propose a nanoantenna based on colloidal silver nanoparticles that has the best fluorescence enhancement coefficient using the example of absorption and fluorescence lines of the TagRFP protein with a low value of intrinsic Y0 (Y0=0.48). It is shown that a fluorophore with a low Y0 value provides a higher fluorescence enhancement value than a fluorophore with a high intrinsic Y0 (Y0=0.8).
Speaker
Sergey V. Zarkov
IPMC RAS; IBPPM RAS
Russia
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