Model and method for optimizing metal-enhanced fluorescence of TagRFP fluorophore molecules taking into account their spectral properties
Sergey V. Zarkov1, Yuri A. Avetisyan1, Garif G. Akchurin1,2, Alexander N. Yakunin1, Valery V. Tuchin1,2,3; 1Institute of Precision Mechanics and Control, Federal Research Centre “Saratov Scientific Centre of the Russian Academy of Sciences”, Saratov, Russia; 2Department of Optics and Biophotonics and Science Medical Center, Saratov State University, Saratov, Russia; 3Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russia
Abstract
The widely used models of metal-enhanced fluorescence (MEF) are traditionally based on the consideration of the parameter values only at discrete two wavelengths of the molecular spectra of the fluorophore: the excitation maximum λex and the emission maximum λem of the molecule. The refined model provides a more realistic description of the interaction of photons of the incident and generated radiation, since both spectra affect the excitation and emission processes of the fluorophore molecule. The features of the influence of the spectral properties of both the nanostructure plasmon and the fluorophore molecule on the efficiency of optimal tuning of fluorescent complexes are studied. Special attention is paid to the procedure for determining the parameters of the "redshift" spectrum of the excitation field intensity gain in the near zone of the plasmonic nanostructure relative to the extinction spectrum, which can be constructed experimentally. An estimate is also given of the influence of the intrinsic quantum yield of the molecule on the integral value of fluorescence enhancement. Numerical results are given using examples of MEF of TagRFP molecules in the vicinity of gold and silver nanorods.
Speaker
Sergey V. Zarkov
IPMC RAS, IBPPM RAS
Russia
Discussion
Ask question
