In Vivo Optoacoustic Angiography of Normal and Diseased Superficial Tissues
Anna G. Orlova1, Ksinia G. Akhmedzhanova1,2, Aleksey A. Kurnikov1, Anna M. Glyavina1,2, Dmitry A. Khochenkov3, Yulia A. Khochenkova3, Anna V. Maslennikova1,2,4, Svetlana V. Nemirova1,4, Ilya V. Turchin1, Pavel V. Subochev1; 1A.V. Gaponov-Grekhov Institute of Applied Physics of RAS, Russia; 2N.I. Lobachevsky State University of Nizhny Novgorod, Russia; 3N.N. Blokhin National Medical Research Center of Oncology, Russia; 4Privolzhsky Research Medical University, Russia
Abstract
Optoacoustic (OA) imaging detects ultrasound waves generated by the absorption of pulsed laser light by endogenous chromophores, enabling label-free vascular imaging at optical penetration depths with ultrasonic resolution. OA imaging is applied in both clinical diagnostics and preclinical studies to assess the structure and function of superficial vascular networks.
In this study, OA was used to examine the vasculature of experimental tumors during growth and after treatment, as well as human skin under normal and pathological conditions. OA angiography was performed using a raster-scan system with a 532 nm laser source and a wideband PVDF detector.
Experiments investigating the effect of the antiangiogenic drug axitinib on experimental tumors revealed a decrease in vascular density and vessel size, accompanying tumor growth inhibition. In studies evaluating tumor response to radiation therapy, OA demonstrated the capability to detect dose-dependent vascular changes associated with different vessel calibers.
In patients with post-thrombotic syndrome of the foot, OA revealed an increase in blood volume, vessel diameter, and tortuosity compared to normal skin, highlighting its diagnostic value in vascular pathologies.
These findings demonstrate the potential of OA angiography for in vivo characterization of normal and pathological vasculature and for non-invasive monitoring of angiogenesis in response to therapy across both preclinical models and clinical applications.
Speaker
Anna Orlova
A.V. Gaponov-Grekhov Institute of Applied Physics of RAS
Russia
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