Investigation of the vascular bed of tumors under the combined effects of antiangiogenic and radiation therapy
Anna M. Glyavina1,2, Daryya A. Voytovich1, Alexey A. Kurnikov1, Yulia A. Khochenkova3,
Dmitry A. Khochenkov3, Anna V. Maslennikova1,2, Ilya .V. Turchin1, Pavel V. Subochev1, Anna G. Orlova1
1- Federal Research Center A.V. Gaponov-Grekhov Institute of Applied Physics of the Russian Academy of Sciences (IAP RAS), Nizhny Novgorod, Russia
2- Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia
3-National Medical Research Center of Oncology named after N.N. Blokhin, Moscow, Russia
Abstract
In this work, using optoacoustic angiography (OA) and diffuse optical spectroscopy (DOS), we evaluated the vascular bed structure and oxygenation levels in experimental neoplasms treated with antiangiogenic and radiation therapies, both individually and in combination.
The study was conducted on Balb/c-nude mice with subcutaneous xenograft of Colo320 (human colon cancer). OA and DOS assessments occurred from day 7 to 36 of tumor growth (3-7 day intervals), with tumor volume measured similarly. Axitinib (VEGFR inhibitor) was administered orally (5 days/week): 25 mg/kg (monotherapy) or 12.5 mg/kg (combination) from days 9 to 33 of tumor growth. Radiation therapy (single dose of 5 Gy) was delivered on day 11.
The OA system comprised a raster-scan configuration with a pulsed laser (532 nm; 1 ns; 2 kHz) and a wideband PVDF detector (IAP RAS). DOS employed a fiber-optic system with a broadband LED source and a spectrometer detector (IAP RAS).
By the end of experiment Axitinib decreased tumor volume 2.4 times, radiation therapy - 4.6 times, and combination therapy - 8.8 times as compared to controls. DOS showed a rapid decrease in StO2 in untreated tumors and during Axitinib therapy. Radiation therapy and the combination caused a transient increase in StO2. OA imaging has shown the decrease of vascular fraction after radiation therapy (alone and in combination).
The work was supported by the Russian Science Foundation, project No. 25-15-00238.
Speaker
Anna Glyavina
1- Federal Research Center A.V. Gaponov-Grekhov Institute of Applied Physics of the Russian Academy of Sciences (IAP RAS), Nizhny Novgorod, Russia 2- Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia
Russia
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