Application of diffuse scattering spectroscopy for intraoperative assessment of blood supply to the gastric tissues
Anna A. Krivetskaya1,2, Daniil M. Kustov1, Vladimir V. Levkin3, Sergey S. Kharnas3, and Tatiana A. Savelieva1,2
1 Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
2 Institute of Engineering Physics for Biomedicine, National Research Nuclear University MEPhI, Moscow, Russia
3 Department of Faculty Surgery No. 1, I.M. Sechenov First Moscow State Medical University, Moscow, Russia
Abstract
Nowadays the evaluation of the state of biological tissues during surgeries is defined by its visible characteristics and therefore depends on the experience of the medical staff. Also it is known that the level of blood supply affects the risk of postoperative complications. Thus there is a necessity of the method which is able to perform objective assessment of the tissue condition.
This work presents methodology which had been designed to estimate the viability of biological tissues by the determination of its oxygen saturation level and degree of blood filling. This technique is based on the processing of diffuse reflected spectra which are recorded by the spectrometer “LESA-01-BIOSPEC”. This work included data collection during operations on 20 patients which had been undergoing surgical interventions with either complete or partial resection of the stomach. In addition, the propagation of the optical radiation in the tissues of gastrointestinal tract was modelled by the Monte-Carlo method.
The tissue condition was assessed at several intraoperative stages: before surgical procedures on the stomach, after mobilization of the vessels, and after the imposition of anastomoses. At the second stage of measurements, the saturation level significantly decreases, while after the imposition of anastomoses in cases of adequate blood supply, the estimated value is restored to its original values. Also, in 6 cases, the blood flow dynamics were assessed after mobilization of the stomach vessels for half an hour. During the entire observation period, the saturation level decreased, but did not fall below 50, which indicates the possibility of tissue function restoration.
Based on the results of the presented work, the developed method of monitoring the level of tissue blood supply by the degree of their oxygen saturation can be used for intraoperative assessment of tissue viability during gastrectomy. The quantitative data obtained during the study determine the objectivity of this method.
The work was conducted within the framework of the state task of the National Research Nuclear University MEPhI (FSWU-2023-0070).
Speaker
Anna Krivetskaya
Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia; Institute of Engineering Physics for Biomedicine, National Research Nuclear University MEPhI, Moscow, Russia
Russia
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