Monitoring Microcirculatory Changes During Photodynamic Therapy
Anastasia V. Guryleva1, Alexander S. Machikhin1, Tatiana G. Grishacheva2, Nikolay N. Petrishchev2
1Scientific and Technological Centre of Unique Instrumentation of RAS, Moscow, Russia
2Pavlov First Saint Petersburg State Medical University, Saint Petersburg, Russia
Abstract
Photodynamic therapy (PDT) is a promising and minimally invasive treatment option for a wide range of diseases. Non-invasive, quantitative, and reliable techniques that can assess tissue responses to photodynamic exposure are in demand for PDT protocols optimization and accurate estimation of treatment efficacy. Alterations in microcirculation—such as reduced perfusion, thrombosis, and increased vascular permeability—can serve as early signs of PDT action. Timely and precise evaluation of microcirculatory parameters allows the adjustment of photosensitizer doses and irradiation settings. This study outlines an experimental setup and digital signal processing algorithms designed to meet this need. With an animal model, we demonstrate the feasibility of simultaneous visualization and quantification of microcirculatory parameters in both tumor and nearby healthy tissues during PDT. The temporal dynamics of several indicators are analyzed throughout photodynamic exposure and after recovery. The limitations of the proposed technique are discussed, along with strategies to improve measurement accuracy.
Speaker
Anastasia Guryleva
STC UI RAS
Russia
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