SILICATE NANOPARTICLES DOPED WITH BIPYRIDYL RUTHENIUM COMPLEXES FOR THE DETERMINATION OF AMITRIPTYLINE BY FLUORESCENT POLARIZING IMMUNOASSAY
Alsina N. Ramazanova1, Elvina P. Medyantseva1, Timur L. Khamidullin1, Marat A. Ziganshin1, Vladimir A. Burilov1, Egor G. Makarov1, Sergei A. Eremin2, Liliya I. Mukhametova2; 1A.M. Butlerov Institute of Chemistry, Kazan (Volga region) Federal University, Kazan, Russian Federation; 2Department of Chemistry, Moscow State University, Moscow, Russian Federation
Abstract
Silicate nanoparticles (diameter – 46 nm) doped with highly luminescent complexes of Ru(II) with bipyridyl, [Ru(dipy)3]Cl2@SiO2, which have high fluorescence intensity, chemical inertia, and the presence of functional groups for binding to antigens, were selected as a fluorescent label for fluorescent polarization immunoassay (FPIA). which can be used by medicinal substances, in particular antidepressants. At was used against tricyclic antidepressants (TCA) and a tracer based on non-covalently amitriptyline-modified silicate NPs. Dynamic light scattering data can serve as confirmation of TCA adsorption: a change in the zeta potential from -48 mV to -37 mV for undoped and from -20.7 to -8 mV for doped silicate NPs. It has been established that against the background of model urine, it is possible to determine amitriptyline in the concentration range of 1E-10 – 1E-5 M with a limit of determination of 8E-10 – 1E-11 M. Since it is possible to immobilize antibodies on the cell surface of polystyrene dies, the At system, a tracer based on silicate nanoparticles doped with highly luminescent bidipyridyl complexes Ru(II) – amitriptyline, amitriptyline in the test sample, can be considered as a biosensor system for the determination of antidepressants using the example of the determination of amitriptyline.
Speaker
Daniil
Kazan Federal University
Russia
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