Detection of Flavonoids Based on Their Complexation with Tb3+ by Surface Enhanced Raman spectroscopy method
Anastasia A. Bartoshevich1, Nikita R. Yarenkov1, Olesya O. Kapitanova1, Irina A. Veselova1; 1Lomonosov Moscow State University, Moscow, Russia.
Abstract
Oxidative stress is the cause of the progress of many serious diseases, such as cancer, atherosclerosis, hypertension, coronary heart disease, diabetes mellitus, etc. Flavonoids found in various plants are natural antioxidants and help inhibit the process of free radical oxidation. In this regard, it is important to control the content of flavonoids, both in biological fluids (blood and urine) and in plant raw materials, which are used for the production of dietary supplements.
Applications in the above areas often require fast and sensitive methods with combination with minimal sample preparation. Spectroscopic methods of analysis, on the basis of which it is possible to create portable devices, are suitable for mass screening of biological fluids and quality control of plant raw materials. Surface-Enhanced Raman Scattering (SERS), as an easy-to-perform and non-destructive method characterized by high selectivity and sensitivity, is a promising approach to the detection of flavonoids. The use of this method, thanks to the instrumentation of portable Raman spectrometers, will make it possible to carry out measurements at the sampling site, which, in turn, contributes to the rapid screening of biological samples.
The formation of complexes of flavonoids and rare-earth metals is a well-known approach to increasing the sensitivity of the determination of plant polyphenols by changing the spectral characteristics of structurally similar compounds. The SERS method in combination with indicator complexation reactions can be used to determine flavonoids in various matrices, due to the significant amplification of the signal, which is due to the use of plasmonic surfaces and changes in the spectral properties of the analytes during coordination.
In this work, the possibility of determining rutin, quercetin and dihydroquercetin using an indicator reaction with Tb3+ ions by the SERS method was studied. The detection limits of rutin and quercetin were 8,5·10-6 M and 5,7·10-5 M, respectively.
Speaker
Anastasia A. Bartoshevich
Lomonosov Moscow State University, Moscow, Russia
Russia
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