Luminescent detection of adenosine-5'-triphosphate using copper nanoclusters
Aleksei A. Furletov1, Vladimir V. Apyari1, Stanislava G. Dmitrienko1; 1Lomonosov Moscow State University, Department of Chemistry
Abstract
The purpose of this study was to develop a simple, rapid and sufficiently selective method for determining adenosine-5'-triphosphate using luminescent copper nanoclusters. Copper nanoclusters were synthesized by reducing copper(II) sulfate with L-ascorbic acid at pH 4 in the presence of poly(N-vinyl-2-pyrrolidone). An original method for determining adenosine-5'-triphosphate in aqueous media was proposed. It was based on selective inhibition of copper nanoclusters luminescence quenching by Cu(II) ions, which is due to the binding of the quencher to the phosphate groups of ATP. The molar concentration of ATP interacting with the quencher was determined by the increase in the copper nanoclusters luminescence intensity at a wavelength of 420 nm. It was found that the maximum value of the analytical signal is achieved in the pH range of 7–9 5 min after the start of the reaction. The detection limit of ATP under the selected conditions is 10 μM, the range of determined contents is from 30 μM to 2 mM. The proposed method for determining adenosine-5'-triphosphate has been successfully applied to the analysis of pharmaceuticals and model systems simulating the composition of wastewater and artificial urine.
The work was supported by the Russian Science Foundation (project No. 24-23-20004).
Speaker
Aleksei A. Furletov
Lomonosov Moscow State University
Russia
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