The therapeutic effect of intrafollicular delivery of vaterite carriers loaded with a glucocorticoid in a psoriasis-like dermatitis in rats in vivo
Mariia S. Saveleva1, Yury I. Surkov1, Polina A. Demina1, Mikhail E. Lobanov1, Isabella A. Serebryakova1, Yulia I. Svenskaya1
1 Saratov State University, Saratov, Russia
Abstract
This work presents a study of the therapeutic effect of topical drug delivery formulation based on vaterite CaCO3 containers with glucocorticosteroid betamethasone dipropionate (CaCO3-BD), on a model of psoriasis-like dermatitis in laboratory rats in vivo.
Psoriasis-like dermatitis was formed in course of daily application of the Keravort cream to experimental skin area at the back of animals for 5 days. After that, the anti-inflammatory therapy with CaCO3-BD containers was performed on the obtained pathological model. The containers were delivered into the hair follicles by means of the sonophoresis. The process of dermatitis formation, as well as the effectiveness of therapy were evaluated using the psoriasis animal-adapted severity index (PASI) according to the degree of erythema, peeling, and coarsening/crusting of the affected area. The daily photofixation of the lesion and monitoring of changes using non-invasive optical methods (diffuse reflectance spectroscopy, optical coherence tomography) were also performed.
As a result of the therapy, a gradual decrease of the disease symptoms in animals was revealed after the start of treatment. It was shown, that the fastest and most effective treatment of animals was provided upon the intrafollicular delivery of the developed CaCO3-BD carriers, as compared with the traditional treatment regimen involving the use of Betamethasone ointment. Importantly, the treatment of animals with the proposed carriers was carried out every 3 days, meaning a total of two treatment procedures per course, meanwhile the ointment was applied daily, twice a day.
Thus, the use of the developed CaCO3-BD carriers form allowed us to reduce the number of treatment procedures by 6 times, while enhancing the therapeutic effect by improving its targeting.
This study was supported by Russian Science Foundation (project № 22-73-10194).
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Mariia Saveleva
Saratov State University
Russian Federation
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