Study of an effect of photo bio modulation on human gut microbiota in vitro under normal conditions and after cryopreservation
Robert N. Khramov1, Liubov V. Zalomova2, Eugeny E. Fesenko (Jr.)2; 1Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow region; 2Institute of Cell Biophysics PSCBR RAS, Pushchino, Moscow region
Abstract
Photobiomodulation (PBM) of the human gut microbiota (HGM) is a fairly new direction in the noninvasive regulation of the body, since HGM plays a role as one of the most important body systems. The effectiveness of PBM the intestinal microbiota in vitro in the system of microorganisms isolated from human stool samples has been studied for the first time. For direct external effects on the microbiota ecosystem through the thickness of the abdominal wall tissues, it is advisable to use light in the range of the biological tissue transparency window (600-1300 nm). Therefore, we compared the efficiency of PBM for two wavelength ranges of LED radiation: in the ranges of red with a maximum of 660 nm and near infrared (NIR) light with a maximum of 940 nm. We tried to obtain a change in the integral parameters: the survival rate of microorganisms before and after cryopreservation, as well as the dynamics of their growth during anaerobic cultivation and pre-irradiation with light at different doses, both for the entire isolated ecosystem of human HGM, as well as for one of the most important species in the HGM family of bifidobacteria (Bifidobacterium breve). It was found that after damage caused by cryopreservation, about half of the initial number of all microorganisms survive, and HGM can significantly change their number in the range from plus 23% to minus 11%, while HGM does not significantly change the survival of cells from the initially isolated microbiota. Similarly, it turned out that the growth dynamics of the initially isolated microorganisms did not change with HGM, and, on the contrary, significantly changed with HGM of microorganisms after cryopreservation. It turned out that in the range of the studied doses from 10 to 67800 J/m2 NIR radiation acts more effectively than PBM with red light. It is shown that the obtained effects of PBM are non-thermal in nature. Thus, it has been shown for the first time that PBM HGM can increase the survival rate of isolated microorganisms after their cryopreservation, as well as change the growth dynamics of cryodamaged HGM in vitro, practically without affecting the initially isolated human microbiota.
Speaker
Robert N. Khramov
Institute of Biophysis PSCBR RAS, Pushchino, Moscow region
Russia
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