Novel approach to the in vivo assessment of BBB integrity and brain tissue clearance with the optofiber photometry
Arseny K. Berdnikov1, Alla V. Stavrovskaya1, Ilia V. Potapenko1, Victoria I. Zhdankina1, Alexandra N. Lukyanchuk1, Yulia K. Komleva1, Vladimir V. Salmin2, Alla B. Salmina; 1Russian Center of Neurology and Neuroscience, Moscow, Russia; 2Bauman Moscow State Technical University, Moscow, Russia
Abstract
Alzheimer's disease (AD) is the most common cause of dementia with complex and multifactorial pathogenesis. Blood-brain barrier (BBB) integrity is significantly compromised in AD. Despite the pivotal importance of the BBB, existing methods for assessing its integrity are limited to post-mortem samples. As a result, the dynamics of BBB impairment development remain undervalued. The method of in vivo BBB integrity assessment could overcome this limitation, providing essential insight to these impairments. Herein we combined intravenous (i.v.) delivery of fluorescent tracer (sodium fluorescein, NaFI) with fiber photometry to analyze BBB integrity in vivo. In this work, we used male mice with a genetic model of AD (5xFAD) and wild-type (WT) mice. We observed a decrease in the fluorescence intensity decay time in 5xFAD mice compared to WT animals following i.v. NaFI injection, indicating altered BBB integrity and brain tissue clearance in AD. We believe this approach can help to elucidate essential details regarding the progression of BBB impairments in AD.
This work was funded by RSF (N 22-15-00126-П).
Speaker
Ilia V. Potapenko
Laboratory of neurobiology and tissue engineering, Russian Сenter of Neurology and Neurosciences
Russia
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