The resistance of brain microvascular endothelial cells to the development of reductive stress in vitro
Novikova S.V.1, Kolotyeva N.A.1, Berdnikov A.K.1, Rozanova N.A.1, Mudarisova R.S.1, Makarov V.I.2, Ryabova A.V.2., Salmina A.B.1
1 Brain Science Institute, Research Center of Neurology, Moscow, Russia
2 Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia
Abstract
The formation of accelerated aging of brain cells during neurodegeneration is associated with the development of reductive stress, characterized by an imbalance between oxidized and reduced forms of pyridine nucleotides. The aim of this study is to investigate the molecular processes occurring in brain microvascular endothelial cells in the context of changes in energy metabolism in vitro. Using modern spectrofluorimetric approaches, particularly FLIM microscopy, new data on the resistance of brain microvascular endothelial cells during the development of reductive stress have been obtained. The results provide a deeper understanding of the mechanisms of aberrant metabolic plasticity occurring in the cells of the neurovascular unit and the blood-brain barrier, which will help identify new therapeutic targets for drug candidates.
Speaker
Novikova Svetlana V.
Brain Science Institute, Research Center of Neurology, Moscow, Russia
Russia
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