Invesigation of method of aberrant protein potentiation using self-assembly of colloidal particles in external rotating fields

Abstract

Neurodegenerative diseases are one of the most significant problems of modern medicine. The development of some of them is associated with the accumulation of aberrant proteins that disrupt the functions of neurons. A large group are synucleinopathies, such as Parkinson's disease, which are characterized by the pathological aggregation of α-synuclein (αSyn). The development of early diagnostic methods through the detection of this aberrant protein is therefore a crucial objective. The general problem is that robust and sensitive techniques for amplifying of pathological αSyn to detectable levels are still needed. Here, we present concept a novel method for aberrant αSyn potentiation based on the controlled self-assembly of colloidal particles in an external rotating electric or conical magnetic field and detecting by fluorescence using the dye thioflavin T. In this paper, we present the methods, designed and assembled experimental systems for potentiation of the aberrant protein αSyn. Optimal parameters for the setups and experimental samples were determined, and experiments were performed with BSA fibrils and cerebrospinal fluid from patients with PD. Based on the experiments, the advantages and disadvantages of the proposed approaches and further development prospects were determined. Thus, a novel concept for potentiation of the aberrant protein αSyn using colloidal self-assembly in an external magnetic field was proposed. This approach will allow the creation of effective test systems for early diagnosis of neurodegenerative diseases based on novel physical phenomena.

Speaker

Anastasiya Shirokova
Bauman Moscow State Technical University, Moscow, Russia
Russia

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