Application of microsecond pulse laser systems for ablation of biological tissues
Polina V. Aleksandrova1, Arsen K. Zotov1, Anna I. Alekseeva2, Yuri A. Suchkov1, David G. Kochiev1, Irina N. Dolganova1,3; 1Prokhorov General Physics Institute of the Russian Academy of Science, Moscow, Russia; 2Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution “Petrovsky National Research Centre of Surgery”, Moscow, Russia; 3Osipyan Institute of Solid State Physics of the Russian Academy of Science, Chernogolovka, Russia
Abstract
Nowadays laser is applied in various fields such as industry, military technology, science and medicine. The attractiveness of its application in clinical practice, especially for surgery and ablation, is due to the minimal hemorrhage, trauma and invasiveness, which can be achieved by using endoscopic and laparoscopic technologies. One of the most important tasks to expand laser applications in medicine is to understand the nature of interaction between laser beam and different types of biological tissues.
In the present work, microsecond pulse laser sources with two different modes of generation were used to compare the exposure of soft tissues to their radiation. Nd:YAG laser with wavelength 1.06 μm was used in the free generation mode, while 〖Nd:YAlO〗_3 laser with wavelength 1.08 μm was used to obtain the pulse train, where each pulse in the train has the duration of 1.2 μs. For this research the Wistar rats’ liver was chosen as a type of soft biological tissue. All experiments were performed in vivo. The duration of the laser radiation in both generation modes was 5 seconds. The assessment of liver tissue damage was performed by histology on the 3-rd and 11-th euthanization days respectively.
The results demonstrated that application of the pulse train as a generation mode for microsecond pulse laser reduces the thermal effects on soft biological tissue when assessing the damage in early period (3-rd day of euthanization) but the analysis of damage on the 11-th euthanization day showed an active regeneration of tissues for both generation modes.
Speaker
Polina Aleksandrova
Prokhorov General Physics Institute of the Russian Academy of Science
Russia
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