Diffuse reflectance spectroscopy for collagen quantification in tumors extracellular matrix
Ryabova A.V.1,2, Romanishkin I.D.1, Vasilieva D.2, Savelieva T.A.1,2, Pominova D.V.1,2
1Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia;
2Institute of Engineering Physics for Biomedicine, National Research Nuclear University MEPhI, Moscow, Russia
Abstract
Desmoplastic stroma, the formation of a dense protective barrier of stromal cells and extracellular matrix (ECM), is a distinctive feature of most advanced carcinomas [1]. Increased tissue stiffness and hydrostatic pressure are significant barriers that limits the therapy. To assess tissue malignancy in breast cancer, diffuse reflectance spectroscopy (DRS), based on the analysis of collagen content, a component of the ECM, can be used [2].
In this work, we used DRS in the shortwave infrared (SWIR) region for noninvasive assessment of ECM density. Absorption spectra from biotissue components and tumor pieces were recorded using a dual-beam spectrophotometer U-3410 (Hitachi, Japan) in the range of 350-2600 nm and a fiber-optic spectrometer DWARF-star (StellarNet Inc., USA) in the range of 975-1700 nm. To calculate the collagen proportion in the sample, we used the spectral unmixing approach to represent the sample spectra as a combination of the primary components’ spectra (water, lipids, nucleic acids, and collagen).
The optimal solution for spectrum approximation by given components was sought in the Microsoft Excel program with the Solver add-in. The minimum value of the Pearson agreement criterion for the sample’s spectrum and its approximation in the range of 975-1700 nm was sought. The developed spectral collagen calculator is a convenient tool for rapid non-invasive assessment of ECM.
The study was supported by a grant from RSF No. 25-12-00436.
1. Cox TR. The matrix in cancer. Nat Rev Cancer. 2021 Apr;21(4):217-238. doi: 10.1038/s41568-020-00329-7.
2. Shanthakumar D. et al. A Comparison of Spectroscopy and Imaging Techniques Utilizing Spectrally Resolved Diffusely Reflected Light for Intraoperative Margin Assessment in Breast-Conserving Surgery: A Systematic Review and Meta-Analysis. Cancers 2023, 15, 2884.
https://doi.org/10.3390/cancers15112884
Speaker
Anastasia Ryabova
GPI RAS
Russia
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