New Fluorescent Labels for Controlled Optical Activation of Platelets
Naumenko M.B.¹, Panfilov M.A.¹², Vorobyev A.Yu.¹², Moskalenksy A.E.¹
¹Novosibirsk State University, Novosibirsk, Russia;
²Vorozhtsov Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
Abstract
Advances in fluorescent molecules have provided powerful means to study biological systems. In the context of platelet research, photolabile (“caged”) compounds - synthetic molecules activated by light - are noteworthy for their ability to selectively activate platelets [1]. Our previous work demonstrated that modified epinephrine analogs linked to photolabile protecting groups via a carbamate bridge enable in vitro studies of platelet activation [2]. In this study, we expand on this approach by using a BODIPY-class dye as the protecting group. Thus, meso-Methyl-BODIPY-conjugated epinephrine compound was synthesized for the first time at the Laboratory of Photoactivated Processes, NIOCH SB RAS. Besides photoactivation, such hybrid BODIPY–epinephrine conjugates exhibit bright fluorescence in the visible range, allowing for platelet visualization.
To preserve platelet integrity and ensure reliable labeling, we optimized protocols for their isolation and staining. A comparative analysis was performed using various anticoagulants, buffer systems, passive sedimentation, and centrifugation methods. We present experimental data on fluorescent labeling and photoactivation of platelets with these compounds. The developed technique broadens the possibilities for controlled studies of platelet activation.
This work was supported by the Ministry of Science and Higher Education of the Russian Federation (project FSUS-2025-0011).
References:
[1] Panfilov, M. A. et al. J. Xenobiotics, 15(1), 8 (2025).
[2] Spiryova, D. V. et al. Biomed. Opt. Express, 11(6), 3319–3330 (2020).
Speaker
Mariia Naumenko
Novosibirsk State University
Russia
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