Integration of Raman Spectroscopy and Principal Component Analysis for Quality Assessment of Omega-3 Fatty Acids

Abstract

Dietary supplements containing omega-3 fatty acids are widely used to address deficiencies and promote health. The quality, quantity and authenticity control of these supplements is of critical importance for effective health maintenance.
Raman spectroscopy is a rapid, non-destructive method for analyzing molecular substances. With advancements in the manufacturing of commercially available portable Raman spectrometers, the demand for the development of effective Raman-based analytical methods has significantly increased. There is a growing trend towards integrating Raman spectroscopy with multivariate statistical methods, enhancing its ability to process large amounts of data and provide detailed insights into complex molecular matters, including heterogeneous systems.`
In this study, the efficiency of both traditional methods and principal component analysis in evaluating the quality of omega-3 fatty acid supplements by Raman spectra is assessed. Specifically, the spectra of triglyceride, ethyl ester, and phospholipid forms of omega-3 fatty acids were recorded and analyzed. The findings demonstrate the ability to successfully differentiate between these forms and perform quantitative analysis of omega-3 fatty acids supplements using Raman spectroscopy.
This study highlights the potential of Raman spectroscopy as a reliable method for the precise and efficient analysis of omega-3 fatty acids. The incorporation of multivariate statistical approaches improves data interpretation, further enhancing accuracy of the method and opening up opportunities for broader applications.
This study was supported by the Russian Science Foundation under the grant № 24-22-20100, https://rscf.ru/project/24-22-20100/.

Speaker

Dmitry D. Vasimov
Prokhorov General Physics Institute of the Russian Academy of Sciences
Russia

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