Development of combined PDT/PTT of model cancer in rats
Elina Nikolaeva1, Elizaveta Diplomatova1, Vadim Genin1,2, Alla Bucharskaya3, Nikita Navolokin3, Boris Khlebtsov4, Valery Tuchin1,2,5, Elina Genina1,2
1 Institution of Physics, Saratov State University, 83, Astrakhanskaya St., Saratov, 410012 Russia
2 Laboratory of Laser Molecular Imaging and Machine Learning, National Research Tomsk State University, 36, Lenin Ave., Tomsk, 634050 Russia
3 Department of Faculty Surgery and Oncology, Saratov State Medical University, 112, Bolshaya Kazachya St., Saratov, 410012 Russia
4 Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences, FRC ”Saratov Science Center of the RAS“, 13, Entuziastov Ave., Saratov, 410049 Russia
5 - Institute of Precision Mechanics and Control, FRC "Saratov Scientific Centre of the RAS", 24, Rabochaya St., Saratov, 410028 Russia
Abstract
The study aimed to develop a combined technology of plasmonic photothermal therapy (PTT) and photodynamic therapy (PDT) in rats with transplanted cholangiocarcinoma РС-1. For PDT, indocyanine green was diluted in polyethylene glycol (PEG) at a ratio of 1:100 and intratumorally injected at a dose of 2 mg/kg. The maximum absorption wavelength of the ICG solution was 790 nm. For PTT, a suspension of PEG-coated gold nanorods (GNR) at a concentration of 400 μg/ml was injected intratumorally into 30% of the tumor volume. The aspect ratio of the GNR was about 4:1, so the plasmon resonance was observed in the spectral range of 800–820 nm. One hour after injections, the tumor was irradiated percutaneously with an 808 nm diode infrared laser at a power density of 2.3 W/cm2 for 15 min. The change in diffuse reflectance spectra of the area under the irradiation as a result of PDT, PTT and combined PDT/PTT of a model rat tumor was investigated. The work was supported by RSF grant No. 23-14-00287.
Speaker
Elina Nikolaeva
Institution of Physics, Saratov State University
Russia
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