Spectral FLIM: addresing endogenous fluorophores with a single wavelength
Vladislav Shcheslavskiy1,2, Boris Yakimov3, Anastasia Komarova2, Eugene Shirshin3, Marina Shirmanova2, Wolfgang Becker1; 1Becker&Hickl GmbH, Germany, 2Privolzhsky Research Medical University, 3M.V. Lomonosov Moscow State University
Abstract
Autofluorescence characteristics of the reduced nicotinamide adenine dinucleotide and oxidized flavin cofactors are important for evaluation of the metabolic status of the cells. The approaches that involve a detailed analysis of both spectral and time characteristics of the autofluorescence signals may provide additional insights in the biochemical processes in the cells and biological tissues, and facilitate the transition of the spectral fluorescence lifetime imaging into clinical applications.
Here, we present the experiments on multispectral fluorescence lifetime imaging with a detailed analysis of the fluorescence decays and spectral profiles of the reduced nicotinamide adenine dinucleotide and oxidized flavin under single excitation wavelength aimed at understanding whether the use of multispectral detection is helpful for metabolic imaging of cancer cells.
Speaker
Vladislav Shcheslavskiy
Privolzhsky Research Medical University
Russia
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