Comparison of LSCI and TR-LSC using Monte Carlo simulation
Arsenii P. Fashchevskii1, Qing Xia2, Yanwen Xu2, Valery V. Tuchin1,3,4, Dan Zhu2, Dongyu Li5; 1Saratov State Univ., Russia; 2Britton Chance Center for Biomedical Photonics- MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics- Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan, Hubei, China; 3Tomsk State Univ., Russia; 4Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the RAS,” Saratov, Russia; School of Optical Electronic Information, Huazhong University of Science and Technology, Wuhan, Hubei, China
Abstract
One of the methods of studying biological tissue using light is laser speckle contrast imaging (LSCI). The essence of the method is to visualize and analyze the speckle pattern that occurs when laser radiation is scattered on small structures of biological tissue. The usual method works with light reflected from an object. This method works well for a relatively thin object or the surface layers of an object. LSCI is used for in vivo blood flow studies. The sizes of microstructures in biological tissues are comparable to the wavelength of incident radiation, therefore, according to the theory of Mie, light is mainly scattered forward. For thicker objects or when examining deeper layers of biological tissue, you can use the transmitted LSCI (TR-LSCI) method. Before proceeding to field experiments, mathematical and computer modeling of such a method of studying biological tissue should be carried out. In this paper, the Monte Carlo method was used to simulate the passage of light. The input parameters are the detector size, the number of layers, the thickness of each layer, the refractive index of each layer, the scattering and reflection coefficients of each layer and the g-factor of each layer. The output data are the coordinates of photons: scattered, absorbed, passed through the object and reflected from it, as well as their number.
The simulation shows that the amount of transmitted light, although small compared to reflected light, carries information about structures located at a greater depth.
Speaker
Fashchevskii Arsenii
Saratov State University
Russia
Discussion
Ask question
