Direct optical generation of singlet oxygen at 1267 nm wavelength stimulates bioenergetics of insulin-producing cells
L.V. Eratova1, I.N. Makovik1, A.Y. Vinokurov1, V.V. Dremin1,2; 1Research & Development Center of Biomedical Photonics, Orel State University, Orel, Russia; 2College of Engineering and Physical Sciences, Aston University, UK
Abstract
Today, diabetes mellitus (DM) affects one in 11 people in the world, so the search for strategies for early treatment of DM is an urgent health problem.
ATP production by mitochondria of β-cells, which is impaired in diabetes, is the main factor of insulin secretion. It seems promising to use activation of mitochondrial respiration and ATP production by β-cell mitochondria as a therapeutic target. Recently, it has been demonstrated that direct laser-induced generation of singlet oxygen (SO) can serve as an activator of mitochondrial respiration and ATP production.
Studies were performed on rat insulinoma RINm5F cells by fluorescence microscopy. SO generation was performed using 1267 nm laser source to excite triplet oxygen.
It was found that:
- 1267 nm laser irradiation activates SO production in cells (with the SO-selective SOSG probe);
- 1267 nm laser irradiation does not lead to the formation of other reactive oxygen species (with superoxide indicator DHE and indicator of mitochondrial ROS MitoTracker Red CM-H2Xros);
- SO leads to a greater increase in NADH production after addition of glucose to cells (NADH autofluorescence studies);
- MMP when glucose is added is higher in irradiated cell group (with a potentiometric TMRM probe);
- SO leads to a decrease in cytosolic Mg2+ production, implying greater mitochondrial ATP production (with the Mag-Fura-2 probe, an indicator of intracellular Mg2+).
The results obtained at this stage indicate the possibility of using laser-induced SC in the regulation of β-cell functions.
The work was supported by RNF grant № 22-75-10088.
Speaker
Lyubov Eratova
Orel State University
Russia
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