A platform for antibacterial photodynamic therapy based on vaterite particles containing a porphyrin derivative as a photosensitizer
A.A. Zakoyan1,2, E.S. Tuchina3, E.S. Prikhozhdenko3, О.I. Guslyakova3, V.V. Tuchin3, O.A. Inozemtseva3; 1Scientific & Production Center “Armbiotechnology”, NAS RA, Yerevan, Armenia; 2Institute of Biochemistry, NAS RA, Yerevan, Armenia; 3Saratov State University, Saratov, Russia
Abstract
The development of biocompatible and biodegradable carriers for the delivery of photosensitizers (PS) is one of the most significant tasks of modern antibacterial photodynamic therapy (APDT) [1]. To increase the efficiency of porphyrin loading, we used calcium carbonate particles containing the sulfonated β-CD. The porphyrin derivative Toet4PyP (PP) was chosen as a PS [2]. The loading efficiency was 44% of the initial PP concentration in the loading solution for ordinary vaterite particles and 83% for sulfo-β-CD-containing particles. We investigated the gradual release of encapsulated PS from vaterite particles into a physiological solution. To study the APDT effect, the obtained particles were dissolved using ethylenediaminetetraacetate (EDTA) and the obtained solutions were investigated against the S. Aureus strain (L.A. Trasevich GISK, Moscow). A LED with a maximum emission spectrum λ=405±20 nm and a power density of 80 mW/cm2 was used as a radiation source. Thus, vaterite particles with a size of 3-3.5 microns containing the sulfo-β-CD molecular complex with a photosensitizer (Toet4PyP) were obtained and investigated as a promising platform for APDT. It was revealed that the use of β-CD provides significant loading efficiency compared to other approaches.
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Anna
Research scientist
Armenia
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