Nanoparticles for SERS detection of formazan
Vladimir A. Mushenkov1, Boris N. Khlebtsov2 , Elena G. Zavyalova1;
1Moscow State University, Moscow, Russia; 2Institute of biochemistry and physiology of plants and microorganisms, Saratov scientific center RAS, Saratov, Russia
Abstract
The resistance of bacterial strains to antibacterial treatment is one of the most important problems in modern medicine. Growing rate of antimicrobial drugs consumption causes antibiotic resistance spreading in the increasing number of bacterial strains. In clinical medicine, commonly used antibiotic susceptibility tests are slow, they require couple of days to obtain results, so empirical therapy schemes are often administered without information of pathogen's resistance. These schemes lead to inappropriate usage of reserve antibiotics or even treatment failure. Previously, we developed a novel rapid method of antibiotic resistance detection based on prokaryotic MTT assay. MTT is a tetrazolium salt which is reduced in living cells to formazan, and the rate of this reaction depends on bacterial viability. If bacterial strain is susceptible to the antibiotic, its addition affects bacterial viability, thus lowering the amount of formazan produced. We use Raman scattering to detect formazan in the sample, which allows quantitative detection without sample preparation or extraction of formazan. This method is rapid, analysis takes 1-2 hours, which is much faster than classical culture based methods. To extend the range of sensitivity of the method we used surface enhanced Raman spectroscopy on gold nanoparticles. In this work, we compared different types of gold nanoparticles, varying their shape and coating, and tested their stability and Raman scattering enhancement both in formazan solution and bacterial samples.
This research was funded by RSCF grant (№ 24-65-00015,
https://rscf.ru/project/24-65-00015/).
Speaker
Mushenkov Vladimir Andreevich
Moscow State University
Russia
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