STUDY OF THE EFFECT OF SYNTHESIS CONDITIONS ON THE SIZE AND STABILITY OF SUBMICRON DROPLETS, DEVELOPMENT OF A TECHNIQUE FOR THE SYNTHESIS OF MULTILAYER MONODISPERSE DROPLETS
Arina V. Papugaeva, 1, Polina G. Rudakovskaya, 1, Tatyana M. Estifeeva, 1,Dmitry A. Gorin, 1
1 Skolkovo Institute of Science and Technology, Moscow, Russia
Abstract
Ultrasound advances research by enhancing visualization and improving drug delivery through mechanical effects like temperature elevation and cavitation. Microbubbles and submicron droplets aid in overcoming physiological barriers, particularly in cancer treatment. Submicron droplets show promise with enhanced properties for better distribution and ultrasound responsiveness, opening new avenues for imaging, therapy, and drug delivery applications.
The work is devoted to the study of the properties of synthesis on the size and stability of submicron droplets based on perftorgexane and bovine serum albumin (BSA). The optimal concentration of BSA was selected for the formation of submicron droplets. The process of coating the obtained submicron droplets with layers using the LbLmethod has been optimized, the optimal ratio of droplets to the applied substance has been selected, and the materials used: tannic acid (TA), poly-L-arginine hydrochloride(PArg), polystyrene sulfonate (PSS) and indocyanine green(ICG). This is how submicron droplets with sequentially applied layers were obtained: TA/(PArg/PSS)6 andTA/(PArg/PSS)3/(PArg/ICG)2(PArg/PSS). The concentration was estimated using flow cytometry, the concentration of submicron droplets after their formation is equal to8*10^7±4*10^6 particles/ml, after applying the layers, the concentration is equal to 3*10^6±10^5 particles/ml. The sizes of submicron droplets were studied using transmission electron microscopy, the size of submicron droplets after their formation is equal to 220±40 nm, and the size after applying the layers is equal to 320±130 nm.
The obtained method provides prospects for studying the physico-chemical properties of multilayer submicron droplets and their subsequent application.
Speaker
Arina V. Papugaeva
Skolkovo Institute of Science and Technology, Moscow, Russia
Russia
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