Pathogen detection using SERS-based aptasensors
Alieva R.Sh.1, Kukushkin V.I.2, Zavyalova E.G.1; 1Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia, 2Institute of Solid State Physics, Russian Academy of Sciences, Chernogolovka, Moscow Region, Russia
Abstract
SERS aptasensors are a promising platform for early disease diagnostics due to their unique combination of high sensitivity and selectivity. DNA aptamers, which are short DNA fragments capable of specifically and high-affinity recognizing target molecules, are used as recognition elements. The modification of nanostructured SERS substrates with aptamers allows creating compact and portable devices for the rapid analysis of biological fluids. This paper presents the results of the development of such devices and their potential application in clinical practice for rapid and accurate detection of biomarkers of various diseases. Aptasensors for detecting influenza viruses and a prototype aptasensor for detecting highly pathogenic E.coli strains are presented as examples. The developed portable devices allow for express analysis of biological fluids in real time, opening up new possibilities for early diagnosis and disease monitoring.
The study was supported by the grant of the Russian Science Foundation No. 24-65-00015,
https://rscf.ru/project/24-65-00015/».
Keywords: aptasensors, SERS (surface-enhanced Raman spectroscopy), nanostructured substrates, biomarkers, early diagnosis, portable devices, express analysis, biological fluids.
Speaker
Rugiyya Aliyeva
Lomonosov Moscow State University
Russia
Discussion
Ask question
