In vivo validation of laser soft tissue reconstruction technology using laser speckle contrast imaging
Dmitry I. Ryabkin1,2, Dmitry D. Stavtsev1,2, Victoria V. Suchkova1,2, Elena A. Morozova3, Gennady A. Pyavchenko2, Alexander Yu. Gerasimenko1,2
1. National Research University of Electronic Technology (MIET), Moscow, Russia
2. I.M. Sechenov First Moscow State Medical University, Moscow, Russia
3. Peoples’ Friendship University of Russia named after Patrice Lumumba, Moscow, Russia
Abstract
The aim of the work is in vivo approbation of the newly developed technology of laser soft tissue reconstruction on laboratory animals.
The laser reconstruction technology involves the application of laser biopolymer solder based on bovine serum albumin protein, indocyanine green dye and carbon nanotubes to the incision and subsequent irradiation of the solder with a diode laser with a wavelength of 810 nm and temperature feedback, which allows continuous control of the heating of the laser seam formation zone.
To demonstrate the efficacy of the technology, an in vivo study was conducted in laboratory rabbits. A total of 28 animals were used, 4 animals for each withdrawal day (1, 3, 5, 10, 30, 60, 90). During the operation and before the day of withdrawal by the method of laser speckle contrast imaging the study of microcirculation restoration of blood flow in the area of laser connection of biological tissues was carried out. After the experiments, histologic and microscopic studies of the laser sutures were performed for each day of withdrawal to evaluate the effectiveness of tissue healing and the degree of scarring, as well as toxicologic studies of the lymph nodes adjacent to the laser reconstruction area.
As a result of the studies, it was determined that the developed technology does not interfere with the blood flow and allows to create joints without the formation of a rough scar, with a suture width 10 times smaller than in the traditional method using needle and thread.
Speaker
Victoria V. Suchkova
National Research University of Electronic Technology (MIET)
Россия
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