RAMAN-BASED OPTICAL AND LIQUID BIOPSY

Abstract

Promising diagnostic methods, such as "optical biopsy" and "liquid biopsy", are in high demand for specific diseases biomarkers detection in biological tissues and fluids. Optical methods have the potential to overcome the limitations of traditional methods of clinical analysis. One of the most promising methods of optical analysis (and optical biopsy) is a Raman spectroscopy, which can contribute to understanding of molecular basis of diseases and creation of new bioanalytical tools for the diagnosis of diseases. Since each type of biological tissue and biofluid has an individual molecular composition and, thus, a unique spectral profile resulting from the transition of a molecule from one vibrational-rotational state to another, a set of such individual states of functional groups of nucleic acids, proteins, lipids and carbohydrates makes it possible to characterize component composition of tissues, which ultimately makes it possible to isolate disease markers. Along with the use of optical biopsy methods, it is possible to apply a supersensitive technique for analyzing biofluids based on surface-enhanced Raman spectroscopy, which will be most effective for detecting low concentrations of disease markers in biological fluids. In the last decade, the development of nanotechnology has led to the creation of promising tools for solving new problems in the study of various human diseases, which is especially important for effective and targeted treatment and a deeper fundamental understanding of the biochemistry of diseases. In this study we demonstrate application of conventional Raman spectroscopy for the analysis of skin tissues and application of SERS for serum analysis to determine the diagnosis of patients with non-communicable diseases.

Speaker

Ivan Bratchenko
Department of Laser and biotechnical systems, Samara National Research University
Russia

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