Experimental study on increasing the penetration depth when treating head tissue with laser using optical clearing
Alaa Sabeeh Shanshool1, E. N. Lazareva2,3 and V. V. Tuchin1,2,3,4,5
1Science Medical Centre, Saratov State University, 410012 Saratov, Russia
2Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk 634050, Russia
3Research Educational Institute of Optics and Biophotonics, Saratov State University, 410012 Saratov, Russia
4A.N. Bach Institute of Biochemistry, FRC “Biotechnology” of the Russian Academy of Sciences, Moscow 119071, Russia
5Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the Russian Academy of Sciences,” 24 Rabochaya str., Saratov 410028, Russia
Abstract
ABSTRACT
Tissue optical clearing (TOC) technology has developed rapidly in the last decade due to advances in microscopic instrumentation and the development of brain imaging systems. This paper presents experimental results on the most important optical properties of rat head tissue, controlled by the introduction of an immersion agent (IA) solution. The refractive index (RI) of rat scalp tissues was measured ex vivo using optical coherence tomography (OCT). The main advantage of this OCT monitoring method is that it allows simultaneous and continuous measurement of both the refractive index and geometric thickness, volume fractions of water and optical clearing agent (OCA) in samples without interrupting the natural course of the OA process. Experiments have shown that the introduction of immersion liquids can effectively control the optical properties of tissues that make (scalp - skull - gray matter - white matter - dura mater) the rat's head more transparent, thereby increasing the ability of light to penetrate the tissue. Immersion of rat head tissue in a solution of 70% glycerol was shown to reduce the light scattering coefficient by approximately 10–15%, where the reflectance spectrum decreased after 60 min. The presented results can be used in the development of functional imaging methods and the development of new methods for diagnosing and treating brain diseases.
Speaker
ALAA SABEEH SHANSHOOL
University Saratov
Iraq
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