Generation and visualization the 3D mice-derived tumor organoids, expressing dcas9-FP probes
Natalia V. Rassomakhina1, Liliya G. Maloshenok1,2, Gerel A. Abushinova 1,2, Victoria V. Zherdeva1,
1Bach Institute of Biochemistry, Federal Research Center of Biotechnology, Russian Academy of Sciences,
2Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, Russia
Abstract
3D tumor organoids are reckoned as the most prominent models to imitating the tumor phenotype and heterogeneity in vivo. Using the different fluorescence molecular probes, especially the genetically encoded probes, may provide the molecular processes targeted visualization [1].
The using of inducible dcas9-FP probes for the in vivo visualization have been reported before [2]. Here we are reporting the tumor organoids generation in vitro based on the tissue resection from the nude mice bearing the tumor. Some approach for their generation, maintaining and fluorescence visualization is discussed.
This work is supported by by the Russian Science Foundation (agreement no. 221400205,
https://rscf.ru/project/22-14-00205/ [in Russian]
References
1.Rassomakhina NV,et al Tumor Organoids: The Era of Personalized Medicine. Biochemistry (Mosc). 2024, 89(Suppl 1):S127-S147
2.Maloshenok LG et al. Tet-Regulated Expression and Optical Clearing for In Vivo Visualization of Genetically Encoded Chimeric dCas9/Fluorescent Protein Probes. Materials (Basel). 2023,16(3):940
Speaker
Rassomakhina Natalia Vadimovna
RC of Biotechnonlgy of the RAS
Russia
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